0023-6152

AA00247166

22581

Unrefereed

(1) Nitrogcous compounds of soy-bean seeds and their transformations during germination were investigated. a) The amount of total nitrogen did not change during germination. b) The amount of nitrogen of basic non-protein compounds in the seed is almost twice as much as that of non-basic nonprotein compounds, but in the seedling tbis proportion reverses though both nitrogens increased. c) The protein in the seeds was isolated almost exbaustively in a pure state; amino acids of the protein hydrolysate were isolated as quantitatively as possible; and several amino-acids, which had not been detected in the hydrolysate of the soy-bean protein, were isolated. d) Organic bases in the water extract of the seeds were isolated. e) Organic bases in the juice of the. seedlings germinated in the dark were isolated, and cadaverine which had not been detected in the higher plant was isolated. A new method for the isolation of cadaverine from a mixture of cadaverine and choline was proposed. f) Asparagine and several monoamino-acids were isolated from the juice of the seedlings germinated in the dark. (2) Transformation of carbohydrates during germination in the dark was examined: although the amount of some carbohydrates increased and that of others decreased, the total amount did not show much change during the author's observation. Starch produced by germination in the dark was isolated, and some of its properties were examined. (3) Variation of oil during germination in the dark was examined. The oil decreased slowly in the early stage, and rapid afterwards ; and there was no evidence of the synthesis of oil during germieation except a chromogenic compound or compounos soluble in ether. (4) Discussions and presumptions in regard to the resulls of the experiments are described.